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KMID : 0900919980220030287
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Korean journal of Animal Reproduction
1998 Volume.22 No. 3 p.287 ~ p.292
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Normal Pregnancy of Mouse Embryos Transferred after Assisted Hatching by a 1.48mutextrm{m} Diode Laser
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Kim Eun-Young
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Abstract
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The objective of this study was to test whether ZP drilling using a 1.48mum diode laser beam on mouse IVF embryos becomes effective the hatching and normal in vivo development, as a preliminary test for obtaining the additional proof that the 1.48mum diode laser could be used safely for human applications. The results obtained in this experiment were as follows: when the hatched rates of mouse embryos by laser ZP drilling according to the embryonic stage were examined until 72 hr (in case of blast tocyst: day 4 after IVF) or 120 hr (in case of 4-cell: day 2 after IVF) after treatment, the d data of laser drilled blastocysts (81.8%) was significantly higher than those of control (hatching blastocyst: day 4 after IVF) (54.2%) and laser drilled 4-cell embryos (45.5%) (p<0.05). When the effect of laser drilling on implantation rates following embryo transfer in day 3 synchronized pseudopregnant recipients was examined, the l laser drilled group (48.7%) was slightly higher than that of control group (43.6%). In addition, when the several pregnant mice delivered in two groups were analysed their chromosomal normality and tested reproductive ability, all p pups were presented normal chromosomal number (n=40) and showed normal growth and reproductive ability. Therefore, these results dem-onstrated that ZP drilling using a 1.48mum diode l laser can increase the embryo hatching and ind duce the normal pregnancy of mouse embryos.
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KEYWORD
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Mouse IVF embryos, ZP drilling, 1.48mutextrm{m} diode laser, Hatching, Implantation
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